Recombinant Human Procalcitonin Protein

Product Discontinued

Recombinant Human Procalcitonin Protein Summary

• Description: A recombinant protein corresponding to 1-116 amino acid residues of human procalcitonin.

  Source: E. coli

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CALCA
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Application Notes: An antigen for immunodetection of human PCT.
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 20 mM Tris-HCl, pH 8.0, 150 mM NaCl
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with deionized water.

Notes

It is recommended to aliquot the product after reconstitution.

Alternate Names for Recombinant Human Procalcitonin Protein

• CALC1
• CALCA
• Calcitonin 1
• calcitonin gene-related peptide 1
• CGRP
• Katacalcin
• Procalcitonin

Background

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CALCA