Human Procalcitonin ELISA Kit (Colorimetric) Summary
| Specificity |
This assay has high sensitivity and excellent specificity for detection of human PCT. No significant cross-reactivity or interference between human PCT and analogues was observed. |
| Standard Curve Range |
7.8 pg/ml-500 pg/ml |
| Sensitivity |
1.95 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10% |
| Intra-Assay |
CV% < 8% |
| Sample Volume |
50-100ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CALCA |
Applications/Dilutions
Reactivity Notes
Please contact our Technical Services with any questions regarding species reactivity
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Human Procalcitonin ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Procalcitonin ELISA Kit (NBP2-60115). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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