Human Procalcitonin ELISA Kit (Colorimetric)

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Summary
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Order Details


    • Catalog Number
      NBP2-60115
    • Availability
      Product Discontinued

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Human Procalcitonin ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human PCT. No significant cross-reactivity or interference between human PCT and analogues was observed.
Standard Curve Range
7.8 pg/ml-500 pg/ml
Sensitivity
1.95 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CALCA

Applications/Dilutions

Dilutions
  • ELISA

Reactivity Notes

Please contact our Technical Services with any questions regarding species reactivity

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human Procalcitonin ELISA Kit (Colorimetric)

  • CALC1
  • CALCA
  • Calcitonin 1
  • calcitonin gene-related peptide 1
  • CGRP
  • Katacalcin
  • Procalcitonin

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Procalcitonin ELISA Kit (NBP2-60115). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol CALCA