Procalcitonin Antibody (4) [Biotin]

Product Discontinued

Procalcitonin Antibody (4) [Biotin] Summary

• Immunogen: Synthetic human alpha-CGRP adsorbed onto aluminum hydroxide gel. The epitope lies within residues 19-37 of human alpha-CGRP1.
• Specificity: This antibody binds human alpha-CGRP. Based on inhibition ELISA, this antibody cross-reacts up to 12% with human beta-CGRP and human amylin, while there is little (<4%) cross-reaction with rat alpha-CGRP.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALCA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

CULTURE MEDIUM: RPMI 1640 with 10% fetal calf serum FUSION PARTNER: SP2mIL6 IMMUNIZATION: Female NMRI x BALB/c mice immunized by intraperitoneal injection

Alternate Names for Procalcitonin Antibody (4) [Biotin]

• CALC1
• CALCA
• Calcitonin 1
• calcitonin gene-related peptide 1
• CGRP
• Katacalcin
• Procalcitonin

Background

Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALCA