PRKRIR Antibody

Western Blot: PRKRIR Antibody [NB100-1555] - Detection of PRKRIR on HeLa whole cell lysate. PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody NB100-1554.

Immunohistochemistry: PRKRIR Antibody [NB100-1555] - Sample: FFPE sections of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PRKRIR used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PRKRIR Antibody [NB100-1555] - FFPE section of human skin basal cell carcinoma. Affinity purified rabbit anti-PRKRIR used at a dilution of 1:250.

Immunohistochemistry-Paraffin: PRKRIR Antibody [NB100-1555] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PRKRIR used at a dilution of 1:250.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PRKRIR Antibody Summary

• Description: Novus Biologicals Rabbit PRKRIR Antibody (NB100-1555) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 150 and 200 of human Protein-Kinase, Interferon-inducible double strand RNA dependent Inhibitor, Repressor of (P58 Repressor) using the numbering given in entry NP_004696.2 (GeneI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THAP12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Control:

  PRKRIR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRKRIR Antibody

• 58 kDa interferon-induced protein kinase-interacting protein
• DAP452 kDa repressor of the inhibitor of the protein kinase
• Death-associated protein 4
• inhibitor of protein kinase PKR
• MGC102750
• P52rIPK
• p58IPK-interacting protein
• protein-kinase, interferon-inducible double stranded RNA dependent inhibitor
• repressor of (P58 repressor)
• THAP domain-containing protein 0
• THAP0

Background

P58IPK is an inhibitor of the serine/threonine protein kinase, PKR. P58IPK functions to repress eukaryotic initiation factor 2-alpha subunit (eIF-2alpha) phosphorylation by PKR. PRKRIR, also designated P52RIPK, is a P58IPK interacting protein that abrogates P58IPK mediated suppression of PKR activity. PRKRIR is also known as 52 kDa repressor of the inhibitor of the protein kinase, p58IPK-interacting protein, 58 kDa interferon-induced protein kinase-interacting protein, P52rIPK, death-associated protein 4, THAP domain-containing protein 0, and MGC102750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THAP12
• Entrez:
  • 5612(Human)
  • 72981(Mouse)
• Uniprot:
  • NP_004696.2(Human)