![Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of PrP on different lysates using anti-PrP antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse brain](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Western-Blot-NBP2-67569-img0006.jpg "Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of PrP on different lysates using anti-PrP antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse brain")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P |
| Clone | SC57-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | C terminal human Prion protein |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PRNP |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Prion protein Antibody (NBP2-67569)Discover more about diseases related to Prion protein Antibody (NBP2-67569).
| Pathways for Prion protein Antibody (NBP2-67569)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRNP |
![Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Prion protein Antibody (SC57-05)-Immunocytochemistry-Immunofluorescence-NBP2-67569-img0007.jpg "Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Immunohistochemistry-Paraffin-NBP2-67569-img0005.jpg "Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded rat brain tissue using anti-PrP antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Flow-Cytometry-NBP2-67569-img0001.jpg "Flow Cytometry: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of SH-SY-5Y cells with PrP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Immunocytochemistry-Immunofluorescence-NBP2-67569-img0002.jpg "Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Immunocytochemistry-Immunofluorescence-NBP2-67569-img0003.jpg "Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded mouse brain tissue using anti-PrP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Prion-protein-Antibody-(SC57-05)-Immunohistochemistry-Paraffin-NBP2-67569-img0004.jpg "Immunohistochemistry-Paraffin: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of paraffin-embedded mouse brain tissue using anti-PrP antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/Doppel-Antibody-Immunohistochemistry-Paraffin-NBP2-31928-img0006.jpg)
![Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize2/Doppel-Antibody-Immunohistochemistry-Paraffin-NBP2-31928-img0005.jpg)
![Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining in human testis and endometrium tissues using anti-PRND antibody. Corresponding PRND RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Doppel Antibody-Immunohistochemistry-Paraffin-NBP2-31928-img0007.jpg)
![Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.](http://images.novusbio.com/fullsize2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-NBP2-13075-img0014.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.](http://images.novusbio.com/fullsize2/beta-Amyloid-Antibody-(MOAB-2)-Immunohistochemistry-Paraffin-NBP2-13075-img0015.jpg)
![Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).](http://images.novusbio.com/fullsize2/beta-Amyloid-Antibody-(MOAB-2)-Western-Blot-NBP2-13075-img0016.jpg)
![Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 6 phosphate isomerasemonoclonal antibody.](http://images.novusbio.com/fullsize/Glucose 6 phosphate isomerase Antibody (2D2)-Western Blot-NBP2-02541-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.](http://images.novusbio.com/fullsize/Glucose 6 phosphate isomerase Antibody (2D2)-Immunocytochemistry-Immunofluorescence-NBP2-02541-img0007.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of paraffin-embedded Human prostate tissue using anti-Glucose 6 phosphate isomerasemouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glucose 6 phosphate isomerase Antibody (2D2)-Immunohistochemistry-Paraffin-NBP2-02541-img0006.jpg)
![Western Blot: GNPDA1 Antibody [NBP2-24557] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 25 ug/ml.](http://images.novusbio.com/fullsize2/GNPDA1 Antibody-Western Blot-NBP2-24557-img0001.jpg)
![Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24557] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize2/GNPDA1-Antibody-Immunohistochemistry-Paraffin-NBP2-24557-img0002.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0005.jpg)
![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg)
![Immunohistochemistry: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Thyroglobulin-Antibody-(2H11+6E1)-Immunohistochemistry-NBP2-34294-img0004.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Immunohistochemistry-Paraffin-NBP2-42388-img0023.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: CD77 Synthase Antibody [NBP2-57614] - Staining of human cell line U-2 OS shows localization to nuclear bodies & mitochondria.](http://images.novusbio.com/fullsize2/CD77 Synthase Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57614-img0001.jpg)
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/14-3-3 Antibody-Western Blot-NBP2-27202-img0001.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Western-Blot-NBP2-25162-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin (NBP1-05208, red) and for DNA (blue). NBP2-25162 stains the neuronal perikarya and processes strongly, and does not stain non neuronal cells in these cultures. The alpha Internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.](http://images.novusbio.com/fullsize2/Tau-Antibody-(2E9)-Immunocytochemistry-Immunofluorescence-NBP2-25162-img0004.jpg)
![Western Blot: ZAK Antibody [NBP1-90367] - Analysis in human cell lines U2OS and MCF-7 using anti-AC013461.1 antibody. Corresponding AC013461.1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/ZAK Antibody-Western Blot-NBP1-90367-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ZAK Antibody [NBP1-90367] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize2/ZAK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90367-img0008.jpg)
![Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-90367] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/ZAK-Antibody-Immunohistochemistry-Paraffin-NBP1-90367-img0009.jpg)
![Western Blot: SPRN Overexpression Lysate (Adult Normal) [NBL1-16429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPRN.](http://images.novusbio.com/fullsize/SPRN Overexpression Lysate (Adult Normal)-Western Blot-NBL1-16429-img0002.jpg)
![EphB1 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "EphB1 [Unconjugated]")
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0032.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Western Blot Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Analysis using the Azide Free version of NB100-56708. Detection of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0037.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - ICC-IF analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section : astocyte and human nuclei. Image from a confirmed customer review.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")