Presenilin-2 Antibody (APS 21)

Western Blot: Presenilin-2 Antibody (APS 21) [NB100-74512] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3).

Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin- 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: Presenilin-2 Antibody (APS 21) [NB100-74512] - Normal biopsies of deparaffinized Human kidney tissue.

Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin- 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin- 2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: APS 21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Presenilin-2 Antibody (APS 21) Summary

• Description: Novus Biologicals Mouse Presenilin-2 Antibody (APS 21) (NB100-74512) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.
• Localization: Endoplasmic reticulum and Golgi Apparatus: endoplasmic reticulum membrane - multi-pass membrane protein; Golgi membrane - multi-pass membrane protein.
• Specificity: Presenilin 2 (APS 21)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSEN2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 4.5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 0.75 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100 - 1:1000
• Application Notes: WB: Detects an approx. 28 kDa protein representing PS2 NT (N-terminus fragment) and approx. 46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, NB100-74512 showed specific Golgi, nuclear envelope, and ER labeling.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Presenilin-2 Antibody (APS 21)

• AD3LP
• AD4AD3L
• AD5
• E5-1
• EC 3.4.23
• EC 3.4.23.-
• presenilin 2 (Alzheimer disease 4)
• Presenilin2
• Presenilin-2
• PS-2
• PS2CMD1V
• PSEN2
• PSNL2
• STM2STM-2

Background

Familial Alzheimer's disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation. PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSEN2
• Entrez:
  • 5664(Human)
  • 19165(Mouse)
  • 81751(Rat)