Presenilin-2 Antibody (APS 21)

Images

 
Western Blot: Presenilin-2 Antibody (APS 21) [NB100-74512] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3).
Immunocytochemistry/ Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin- 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunohistochemistry-Paraffin: Presenilin-2 Antibody (APS 21) [NB100-74512] - Normal biopsies of deparaffinized Human kidney tissue.
Immunocytochemistry/ Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more
Immunocytochemistry/ Immunofluorescence: Presenilin-2 Antibody (APS 21) [NB100-74512] - Presenilin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
APS 21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Presenilin-2 Antibody (APS 21) Summary

Immunogen
Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.
Localization
Endoplasmic reticulum and Golgi Apparatus: endoplasmic reticulum membrane - multi-pass membrane protein; Golgi membrane - multi-pass membrane protein.
Specificity
Presenilin 2 (APS 21)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSEN2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • ELISA 4.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.75 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
WB: Detects an approx. 28 kDa protein representing PS2 NT (N-terminus fragment) and approx. 46 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed COS-7 cells transfected with hPS2, NB100-74512 showed specific Golgi, nuclear envelope, and ER labeling.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Presenilin-2 Antibody (APS 21)

  • AD3LP
  • AD4AD3L
  • AD5
  • E5-1
  • EC 3.4.23
  • EC 3.4.23.-
  • presenilin 2 (Alzheimer disease 4)
  • Presenilin2
  • Presenilin-2
  • PS-2
  • PS2CMD1V
  • PSEN2
  • PSNL2
  • STM2STM-2

Background

Familial Alzheimer's disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation. PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Presenilin-2 Antibody (NB100-74512) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-74512AF405
Alexa Fluor 488 NB100-74512AF488
Alexa Fluor 647 NB100-74512AF647
Alexa Fluor 700 NB100-74512AF700
DyLight 350 NB100-74512UV
DyLight 405 NB100-74512V
DyLight 488 NB100-74512G
DyLight 680 NB100-74512FR
DyLight 755 NB100-74512IR

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Bioinformatics

Gene Symbol PSEN2
Entrez