Presenilin-1 Overexpression Lysate

Western Blot: Presenilin 1 Overexpression Lysate (Adult Normal) [NBL1-14860] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Presenilin 1.

• Reactivity: Hu
• Applications: WB

Presenilin-1 Overexpression Lysate Summary

• Description:

  Presenilin-1 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC216443
  
  Accession#: NM_000021
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: PSEN1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 52.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Presenilin-1 Overexpression Lysate

• AD3EC 3.4.23.-
• Alzheimer disease 3
• EC 3.4.23
• FAD
• presenilin 1
• Presenilin1
• Presenilin-1
• Protein S182
• PS-1
• PS1presenilin-1
• PSEN1
• PSNL1
• S182

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (I-467). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Presenilin-1 Lysate (NBL1-14860). (Showing 1 - 1 of 1 FAQ).

1. I purchased your product Presenilin 1 Lysate (NBL1-14860) this year. I would like to know if you could tell me the exact concentration of Presenilin-1 present in such lysate, since the concentration given (1mg/ml) is for the total protein content. It would be really helpful for our experiments ongoing at the moment regarding AD.
   • The Presenilin 1 lysate was produced in HEK293T cells using plasmid ID RC216443. The concentration of the lysate protein is 1mg/ml however, I'm unable to confirm the exact amount or concentration of the overexpressed protein. The protein contains a C-terminal DDK tag and its presence in the lysate was confirmed by Western blot.

Bioinformatics

• Gene Symbol: PSEN1