Presenilin-1 Antibody [Unconjugated] - C-terminal Fragment

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Presenilin-1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Presenilin-1 C-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Presenilin-1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Presenilin-1 Antibody [Unconjugated] - C-terminal Fragment Summary

• Immunogen: E. coli-derived recombinant human Presenilin-1
  aa 298-407
• Specificity: Detects human Presenilin‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Presenilin‑1 N-Terminal Fragment (aa 1‑80), Presenilin‑2 C‑Terminal Fragment (aa 322‑388), and rhPresenilin‑2
  N‑Terminal Fragment (aa 1‑80) is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSEN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Presenilin-1 Antibody [Unconjugated] - C-terminal Fragment

• AD3EC 3.4.23.-
• Alzheimer disease 3
• EC 3.4.23
• FAD
• presenilin 1
• Presenilin1
• Presenilin-1
• Protein S182
• PS-1
• PS1presenilin-1
• PSEN1
• PSNL1
• S182

Background

Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Presenilin-1 Antibody (AF166)(1)

Publication using AF166

• J Sidor, M Gillette, LA Dezi, G Untiveros, L Strizzi Role of Presenilin-1 in Aggressive Human Melanoma International Journal of Molecular Sciences, 2022-04-28;23(9):. 2022-04-28 [PMID: 35563300] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PSEN1