![Western Blot: PRAGMIN Antibody [NBP1-42689] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pragmin antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pragmin was also immunoprecipitated by another rabbit anti-Pragmin antibody , which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/PRAGMIN-Antibody-Western-Blot-NBP1-42689-img0002.jpg "Western Blot: PRAGMIN Antibody [NBP1-42689] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pragmin antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Pragmin was also immunoprecipitated by another rabbit anti-Pragmin antibody , which recognizes a downstream epitope.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | Novus Biologicals Rabbit PRAGMIN Antibody (NBP1-42689) is a polyclonal antibody validated for use in WB and IP. Anti-PRAGMIN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 275 and 325 of human homolog of rat pragma of Rnd2 using the numbering given in entry NP_001074295.1 (GeneID 157285). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SGK223 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot DOK1 [p Tyr362] Antibody - BSA Free](https://images.novusbio.com/images/DOK1-[p-Tyr362]-Antibody-Western-Blot-NB100-82112-img0001.jpg)
![Immunohistochemistry-Paraffin DOK1 [p Tyr362] Antibody - BSA Free](https://images.novusbio.com/images/nb100-82112_rabbit-polyclonal-dok1-p-tyr362-antibody-103202523464515.jpg)
![Western Blot DOK1 [p Tyr362] Antibody - BSA Free](https://images.novusbio.com/images/DOK1-[p-Tyr362]-Antibody-Western-Blot-NB100-82112-img0004.jpg)
| Publication using NBP1-42689 | Applications | Species |
|---|---|---|
| Xu J, Wan M, He Q et al. SGK3 is associated with estrogen receptor expression in breast cancer. Breast Cancer Res Treat 2012-07-01 [PMID: 22576469] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRAGMIN Antibody (NBP1-42689)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![Western Blot GRB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GRB2_AF3846_Western_Blot_5355.jpg)
![Immunohistochemistry GRB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GRB2_AF3846_Immunohistochemistry_6809.jpg)
![Western Blot EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Knockout_Validated_21256.jpg)
![Immunohistochemistry EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Immunohistochemistry_7327.jpg)
![Flow Cytometry EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Flow_Cytometry_20077.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")