PRAF1 Antibody

Images

 
Western Blot: PRAF1 Antibody [NBP1-56449] - Lanes: Lane1: 50 ug mouse heptoma cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: PORL1E
Western Blot: PRAF1 Antibody [NBP1-56449] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Hela cell lysate

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PRAF1 Antibody Summary

Immunogen
Synthetic peptides corresponding to POLR1E (polymerase (RNA) I polypeptide E, 53kDa) The peptide sequence was selected from the N terminal of POLR1E)(50ug). Peptide sequence SPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTL.
Specificity
This product is specific to Subunit or Isoform: RPA49.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR1E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against POLR1E and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for PRAF1 Antibody

  • DNA-directed RNA polymerase I subunit E
  • DNA-directed RNA polymerase I subunit RPA49
  • FLJ13390
  • FLJ13970
  • FLJ43482
  • PAF53RNA polymerase I-associated factor 1
  • polymerase (RNA) I associated factor 1
  • polymerase (RNA) I polypeptide E, 53kDa
  • PRAF1
  • RNA polymerase I associated factor 53
  • RNA polymerase I subunit A49
  • RNA polymerase I-associated factor 53
  • RP11-405L18.3

Background

POLR1E is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.POLR1E is the component of RNA polymerase I which synthesizes ribosomal RNA precursors.POLR1E appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POLR1E
Uniprot