PQBP1 Antibody - BSA Free

Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry: PQBP1 Antibody [NBP1-47270] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:1,000 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PQBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PQBP1 Antibody - BSA Free (NBP1-47270) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 150 and 200 of human Polyglutamine Binding Protein 1 using the numbering given in entry NP_005701.1 (GeneID 10084).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PQBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate.
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PQBP1 Antibody - BSA Free

• 38 kDa nuclear protein containing a WW domain
• MRX55
• MRXS3
• MRXS8
• Npw38
• NPW38mental retardation, X-linked 55
• nuclear protein containing WW domain 38 kD
• polyglutamine binding protein 1
• Polyglutamine tract-binding protein 1
• polyglutamine-binding protein 1
• PQBP-1
• RENS1
• SHS
• Sutherland-Haan X-linked mental retardation syndrome

Background

PQBP1 is a nuclear polyglutamine-binding protein that contains a WW domain (Waragai et al., 1999 [PubMed 10332029]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PQBP1
• Entrez:
  • 10084(Human)