WBP11 Antibody - BSA Free

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded rat brain. WBP11 antibodydiluted at 1:500.

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded mouse brain. WBP11 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded rat adrenal. WBP11 antibody diluted at 1:500.

Immunoprecipitation: WBP11 Antibody [NBP2-20886] - Jurkat whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of WBP11 protein by 4 ug WBP11 antibody 7.5 % SDS-PAGE The immunoprecipitated WBP11 protein was detected by WBP11 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Western Blot: WBP11 Antibody [NBP2-20886] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with WBP11 antibody (NBP2-20886) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WBP11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WBP11 Antibody - BSA Free (NBP2-20886) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human WBP11. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm
• Predicted Species: Bovine (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WBP11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for WBP11 Antibody - BSA Free

• DKFZp779M1063
• Npw38-binding protein NpwBP
• Npw38-binding protein
• NpwBP
• NPWBPsplicing factor, PQBP1 and PP1 interacting
• SH3 domain-binding protein SNP70
• SIPP1WW domain-binding protein 11
• SNP70
• Splicing factor that interacts with PQBP-1 and PP1
• WBP-11
• WW domain binding protein 11

Background

This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for WBP11 Antibody (NBP2-20886) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/06/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 40835

Bioinformatics

• Gene Symbol: WBP11
• Uniprot:
  • Q9Y2W2(Human)