WBP11 Antibody

Images

 
Western Blot: WBP11 Antibody [NBP2-20886] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with WBP11 antibody diluted by 1:2000.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded rat brain. WBP11 antibodydiluted at 1:500.
Western Blot: WBP11 Antibody [NBP2-20886] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded mouse brain. WBP11 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: WBP11 Antibody [NBP2-20886] - Paraffin-embedded rat adrenal. WBP11 antibody diluted at 1:500.
Immunoprecipitation: WBP11 Antibody [NBP2-20886] - Jurkat whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of WBP11 protein by 4 ug WBP11 antibody 7.5 % SDS-PAGE The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MkSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Order Details

WBP11 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WBP11. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Predicted Species
Bovine (99%), Monkey (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WBP11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20886 in the following applications:

Reactivity Notes

Rhesus Monkey (99%), Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WBP11 Antibody

  • DKFZp779M1063
  • Npw38-binding protein NpwBP
  • Npw38-binding protein
  • NpwBP
  • NPWBPsplicing factor, PQBP1 and PP1 interacting
  • SH3 domain-binding protein SNP70
  • SIPP1WW domain-binding protein 11
  • SNP70
  • Splicing factor that interacts with PQBP-1 and PP1
  • WBP-11
  • WW domain binding protein 11

Background

This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WBP11 Antibody (NBP2-20886) (0)

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Review for WBP11 Antibody (NBP2-20886) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-20886:
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WB
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Images Ratings Applications Species Date Details
Western Blot WBP11 NBP2-20886
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reviewed by:
Anonymous
WB Human 06/06/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40835

Blocking

Blocking DetailsBlocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP linked
Secondary Manufacturer Cat#7074P2
Secondary Concentration1:20,000

Details

Detection Notes20 seconds exposure

Comments

CommentsPerfect antibody for WBP11 protein.

Product General Protocols

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FAQs for WBP11 Antibody (NBP2-20886) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
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3
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2
0
1
0

Anonymous
06/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol WBP11
Uniprot