PPP2R5A Antibody

Western Blot: PPP2R5A Antibody [NB100-847] - Staining of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R5A Antibody Summary

• Immunogen: Peptide with sequence C-AYNMHSILSNTSAE corresponding to C-Terminus according to NP_006234.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP2R5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: WB: Approx. 55 kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2 kDa band according to NP_006234.1). IHC-P: Human heart shows textured cytoplasm staining in cardiomyocytes.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R5A Antibody

• B56A
• MGC131915
• PP2A B subunit isoform B56-alpha
• PP2A B subunit isoform B'-alpha
• PP2A B subunit isoform PR61-alpha
• PP2A B subunit isoform R5-alpha
• PP2A, B subunit, B' alpha isoform
• PP2A, B subunit, B56 alpha isoform
• PP2A, B subunit, PR61 alpha isoform
• PP2A, B subunit, R5 alpha isoform
• PR61A
• PR61alpha
• protein phosphatase 2, regulatory subunit B (B56), alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alphaisoform
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Background

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R5A Antibody (NB100-847)(1)

Publication using NB100-847

• McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995-11-03 [PMID: 7592815]

Bioinformatics

• Gene Symbol: PPP2R5A
• Entrez:
  • 5525(Human)
• Uniprot:
  • Q15172(Human)