PP2C gamma/PPM1G Antibody - Azide and BSA Free

Western Blot: PP2C gamma/PPM1G Antibody [NBP2-99758] - Anti-PP2C gamma/PPM1G rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 292T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 80 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: PP2C gamma/PPM1G Antibody [NBP2-99758] - PP2C gamma/PPM1G was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-PP2C gamma/PPM1G rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PP2C gamma/PPM1G rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PP2C gamma/PPM1G Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human PP2C gamma/PPM1G (Accession#: O15355; Met 317-Asp 546)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1G
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 4-6 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PP2C gamma/PPM1G Antibody - Azide and BSA Free

• EC 3.1.3.16
• MGC1675
• MGC2870
• PP2C gamma
• PP2C, gamma
• PP2CG
• PP2CGAMMA
• PP2C-gamma
• PPM1C
• PPM1G
• PPP2CG
• Protein phosphatase 1C
• protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• protein phosphatase 1G
• protein phosphatase 2, catalytic subunit, gamma isoform
• protein phosphatase 2C gamma isoform
• Protein phosphatase 2C isoform gamma
• Protein phosphatase magnesium-dependent 1 gamma
• protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1G