PP2C gamma/PPM1G Antibody

Western Blot: PP2C gamma/PPM1G Antibody [NB110-38867] - Detection of Human PPM1G on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T (50 microgram) using NB110-38867. PPM1G was also immunoprecipitated by rabbit anti-PPM1G antibody NB110-38869.

Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:500.

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody [NB110-38867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-PPM1G used at a dilution of 1:100.

Immunohistochemistry: PP2C gamma/PPM1G Antibody [NB110-38867] - Sample: FFPE sections of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PPM1G used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PP2C gamma/PPM1G Antibody Summary

• Description: Novus Biologicals Rabbit PP2C gamma/PPM1G Antibody (NB110-38867) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-PP2C gamma/PPM1G Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Protein Phosphatase Magnesium-dependent 1 Gamma using the numbering given in entry NP_817092.1 (GeneID 5496).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody can be used for immunoprecipitation and Western blot analysis. Prepare working dilution immediately before use.
• Control:

  HeLa Whole Cell Lysate
  PP2C gamma/PPM1G Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PP2C gamma/PPM1G Antibody

• EC 3.1.3.16
• MGC1675
• MGC2870
• PP2C gamma
• PP2C, gamma
• PP2CG
• PP2CGAMMA
• PP2C-gamma
• PPM1C
• PPM1G
• PPP2CG
• Protein phosphatase 1C
• protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• protein phosphatase 1G
• protein phosphatase 2, catalytic subunit, gamma isoform
• protein phosphatase 2C gamma isoform
• Protein phosphatase 2C isoform gamma
• Protein phosphatase magnesium-dependent 1 gamma
• protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C gamma/PPM1G Antibody (NB110-38867)(3)

Publications using NB110-38867

• Huang W, Chen X, Wu H et al. PPM1G promotes chemoresistance in triple negative breast cancer by enhancing YAP signaling. Pharmacological research 2025-10-08 [PMID: 41072836]
• Hutten S, Prescott A, James J et al. An intranucleolar body associated with rDNA. Chromosoma 2011-10-01 [PMID: 21698343]
• Ryan K, Khleborodova A, Pan J et al. Small molecule activators of pre-mRNA 3' cleavage. RNA 2009-03-01 [PMID: 19155323]

Bioinformatics

• Gene Symbol: PPM1G
• Entrez:
  • 5496(Human)
• Uniprot:
  • NP_817092.1(Human)