PP2C beta/PPM1B Antibody

Western Blot: PP2C beta/PPM1B Antibody [NB110-38886] - PPM1B Antibody [NB110-38886] - Detection of Human and Mouse PPM1B on HeLa whole cell lysate using NB110-38886. PPM1B was also immunoprecipitated by rabbit anti-PPM1B antibody NB110-38885.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PP2C beta/PPM1B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 479) of human Protein Phosphatase 1B, Magnesium-Dependent, Beta Isoform using the numbering given in entry NP_002697.1 (GeneID 5495).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PP2C beta/PPM1B Antibody

• EC 3.1.3.16
• MGC21657
• PP2C beta
• Pp2c2
• PP2CB
• PP2C-beta
• PP2C-beta-X
• PP2CBmagnesium-dependent, beta isoform
• PPM1B
• protein phosphatase 1B
• Protein phosphatase 2C isoform beta
• protein phosphatase 2C-like protein
• protein phosphatase, Mg2+/Mn2+ dependent, 1B

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C beta/PPM1B Antibody (NB110-38886)(1)

Publication using NB110-38886

• Yien YY, Bieker JJ. Functional interactions between erythroid Kruppel-like factor (EKLF/KLF1) and protein phosphatase PPM1B/PP2Cbeta. J Biol Chem 2012-05-01 [PMID: 22393050]

Bioinformatics

• Gene Symbol: PPM1B
• Entrez:
  • 5495(Human)
• Uniprot:
  • NP_002697.1(Human)