PP2C alpha/PPM1A Antibody - Azide and BSA Free

Western Blot: PP2C alpha/PPM1A Antibody [NBP3-00058] - Anti-PP2C alpha/PPM1A rabbit polyclonal antibody at 1:2000 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 44 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP3-00058] - Immunochemical staining of human PP2C alpha/PPM1A in human colon carcinoma with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP3-00058] - Immunochemical staining of human PP2C alpha/PPM1A in human breast carcinoma with rabbit polyclonal antibody at 1:3000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: PP2C alpha/PPM1A Antibody [NBP3-00058] - PP2C alpha/PPM1A was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate 4 ul anti-PP2C alpha/PPM1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PP2C alpha/PPM1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PP2C alpha/PPM1A Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human PP2C alpha/PPM1A (Accession#: P35813-1; Met1-Trp382)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPM1A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PP2C alpha/PPM1A Antibody - Azide and BSA Free

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1A