PP2A alpha Antibody (H5D12) - BSA Free

Western Blot: PPP2R4 Antibody (H5D12) [NBP2-29818] - This lot detected PP2A, B subunit, B56 delta in HeLa nuclear cell extract when it was tested at 1:2000 in blocking buffer.

• Reactivity: Hu
• Applications: WB
• Clone: H5D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PP2A alpha Antibody (H5D12) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_006245
• Immunogen: GST fusion protein corresponding to the N-terminus of human PP2A, B subunit, B56 delta.
• Specificity: Human PP2A, B subunit, B56 delta. This monoclonal antibody is the delta isoform for the B56 family of protein phosphatase 2A.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP2CA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_006245

Alternate Names for PP2A alpha Antibody (H5D12) - BSA Free

• EC 3.1.3.16
• PP2A
• PP2A-alpha
• PP2Ac
• PP2CA
• PP2Calpha
• PPP2CA
• protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• protein phosphatase 2, catalytic subunit, alpha isozyme
• protein phosphatase 2A catalytic subunit, alpha isoform
• Replication protein C
• RP-C
• serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
• serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma. The B' (or B56) family consists of at least seven splice variantsderived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP2CA
• Uniprot:
  • Q14738(Human)