Human PP14/Glycodelin ELISA Kit (Colorimetric)

ELISA: Human PP14/Glycodelin ELISA Kit (Colorimetric) [NBP2-82133] - Samples were spiked with high concentrations of Human PP14/Glycodelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human PP14/Glycodelin ELISA Kit (Colorimetric) [NBP2-82133] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Milk, Cell Supernatant
• Standard Curve Range: 4.69 - 300 ng/mL
• Sensitivity: 2.81 ng/mL

Human PP14/Glycodelin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human PP14/Glycodelin in samples. No Significant cross-reactivity or interference between Human PP14/Glycodelin and analogues was observed.
• Standard Curve Range: 4.69 - 300 ng/mL
• Sensitivity: 2.81 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 6%
• Spike Recovery: average = 96% for serum; average = 98% for EDTA plasma; average = 102% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PAEP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PP14/Glycodelin ELISA Kit (Colorimetric)

• alpha uterine protein
• GdF
• GdS
• Glycodelin
• glycodelin-A
• glycodelin-F
• glycodelin-S
• MGC138509
• MGC142288
• PAEP
• PEP
• Placental protein 14
• PP14 protein (placental protein 14)
• PP14
• PP14GDPAEGGdA
• pregnancy-associated endometrial a
• Pregnancy-associated endometrial alpha-2 globulin
• pregnancy-associated endometrial alpha-2-globulin
• progestagen-associated endometrial protein (placental protein 14
• progestagen-associated endometrial proteinPEG
• Progesterone-associated endometrial protein

Background

PAEP (Progesterone-associated endometrial protein, glycodelin, PEG, PP14) is a glycoprotein belonging to lipocalin structural superfamily. It shares a sequence homology to beta-lactoglobulins containing a retinol-binding motif and it is mainly produced by secretory and decidualized endometrium in women and by seminal vesicle epithelium in men (1). PAEP function is not clearly understood. However, glycosylated version of PAEP (GdA) has been associated to contraceptive and immunosuppressive activities during reproduction. PAEP is the main protein produced by secretory endometrium during mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Therefore, PAEP has been linked as an ideal biomarker of endometrial activity and function for in vitro fertilization treated women (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PP14/Glycodelin ELISA Kit (NBP2-82133). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PAEP