PP1 Antibody (319319)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit Monoclonal Antibody (Catalog # MAB3000) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PP1 catalytic subunits at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 319319
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PP1 Antibody (319319) Summary

• Immunogen: E. coli-derived recombinant human PP1 alpha catalytic subunit
  Lys41-Pro298
  Accession # P62136
• Specificity: Detects endogenous human, mouse and rat PP1 alpha . Based on the amino acid homologies, the antibody is expected to cross‑react with PP1 beta and PP1 gamma .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1CA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP1 Antibody (319319)

• PP1
• PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP1 Antibody (MAB3000)(2)

Publications using MAB3000

• Boratko A, Gergely P, Csortos C Cell cycle dependent association of EBP50 with protein phosphatase 2A in endothelial cells. PLoS ONE, 2012-04-16;7(4):e35595. 2012-04-16 [PMID: 22523604] (Western Blot, Bovine)
• Csortos C, Czikora I, Bogatcheva NV, Adyshev DM, Poirier C, Olah G, Verin AD TIMAP is a positive regulator of pulmonary endothelial barrier function. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-06-27;295(3):L440-50. 2008-06-27 [PMID: 18586956] (ICC, Human)

Bioinformatics

• Gene Symbol: PPP1CA
• Uniprot:
  • P62136(Human)