PP1 alpha/PPP1A Antibody - BSA Free

Western Blot: PP1 alpha/PPP1A Antibody [NBP3-30764] - Legend: Novus Biologicals' rabbit anti PP 1 alpha antibody western blot of total rat brain homogenate.(+) indicates staining done in the presence of specific blocking peptide or (-) peptide control. The darkest mark is located at -36-38 kDa.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PP1 alpha/PPP1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PP1 alpha/PPP1A Antibody - BSA Free (NBP3-30764) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate the purified antibody was a peptide conjugated to KLH corresponding to the sequence NH2-Arg-Pro-Ile-Thr-Pro-Pro-Arg-Asn-Ser-AlaLys-Ala-Lys-Lys-COOH. This peptide antibody corresponds to C -terminal peptide of PP1 alpha/PPP1A catalytic subunit having a MW of 38kDa. The sequence used is amino acid 317-330.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1CA
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot : 1:500-1:1000
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for PP1 alpha/PPP1A Antibody - BSA Free

• EC 3.1.3.16
• PP1 alpha
• PP1alpha
• PP-1AMGC15877
• PPP1A
• PPP1AMGC1674
• PPP1CA
• protein phosphatase 1, catalytic subunit, alpha isoform
• protein phosphatase 1, catalytic subunit, alpha isozyme
• serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
• serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1CA