POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

Staining of U-251 cells using the Anti-POU3F2/OCT7 monoclonal antibody) .

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2/OCT7 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of mouse brain shows moderate nuclear positivity in neurons in cortical layers 2-4.

Analysis in human cell line U-251 MG.

Analysis in human cerebral cortex tissue.

Staining of mouse forebrain shows nuclear positivity in a subset of cells in the lateral ventricle wall.

Analysis in mouse cerebral cortex tissue.

Staining of mouse brain shows no nuclear positivity in neurons in cortical layers 5-6 as expected.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: CL6228
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P20265, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POU3F2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: PFA/Triton X-100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

• Brain-2
• Brain-specific homeobox/POU domain protein 2
• BRN2
• brn-2
• BRN2brain-2
• Nervous system-specific octamer-binding transcription factor N-Oct-3
• Oct7
• oct-7
• OCT7POU domain class 3, transcription factor 2
• Octamer-binding protein 7
• Octamer-binding transcription factor 7
• OTF7
• OTF-7
• POU class 3 homeobox 2
• POU domain, class 3, transcription factor 2
• POU3F2
• POUF3

Background

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU3F2