Porcine IL-12/IL-23 p40 Quantikine ELISA Kit

• Reactivity: Po
• Applications: ELISA
• Conjugate: HRP

Porcine IL-12/IL-23 p40 Quantikine ELISA Kit Summary

• Background: The Quantikine Porcine IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure porcine IL-12/IL-23 p40 in cell culture supernates, serum and plasma. It contains CHO cell-expressed recombinant porcine IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natura...l porcine IL-12/IL-23 p40 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring porcine IL-12/IL-23 p40.
  Show More
• Specificity: Natural and recombinant porcine IL-12/IL-23 p40
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Protein/Peptide Type: Peptide
• Gene: MGST1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine IL-12/IL-23 p40 Quantikine ELISA Kit

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-12/IL-23 p40 (P1240)(5)

Publications using P1240

• Li, Q;Jiang, Y;Zheng, M;Sun, X;Hui, L;Zhang, Y;Yue, H;Qi, Y;Li, S;Ke, J;Li, Q;Ma, B;Jia, X;Wang, F;Mi, L;Zhang, S;Miao, F;Wang, S;Zhang, F;Chen, T;Hu, R; Newcastle Disease Virus-Vectored African Swine Fever Virus Antigen Cocktail Delays the Onset of ASFV-SY18 but Is Not Protective Microorganisms 2024-12-13 [PMID: 39770792] (Porcine)
• Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis. Crit. Care Med., 2010-06-01;38(6):1467-74. 2010-06-01 [PMID: 20400898] (Porcine)
• Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J., 2009-10-19;24(3):712-22. 2009-10-19 [PMID: 19841036] (Porcine)
• Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues. Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401. 2009-04-08 [PMID: 19357220] (Porcine)
• Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model. J. Immunol., 2008-05-01;180(9):6346-53. 2008-05-01 [PMID: 18424758] (Porcine)

FAQs for IL-12/IL-23 p40 (P1240). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MGST1