Porcine IL-12/IL-23 p40 DuoSet ELISA, 15 Plate

• Reactivity: Po
• Applications: ELISA
• Conjugate: Biotin

Porcine IL-12/IL-23 p40 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porcine IL-12/IL-23 p40 DuoSet ELISA, 15 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12/IL-23 p40 (DY912)(8)

Publications using DY912

• B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation Microorganisms, 2021-08-28;9(9):. 2021-08-28 [PMID: 34576723] (Porcine)
• K Obremski, W Trybowski, P Wojtacha, M Gaj?cka, J Tyburski, ? Zielonka The Effect of Zearalenone on the Cytokine Environment, Oxidoreductive Balance and Metabolism in Porcine Ileal Peyer's Patches Toxins (Basel), 2020-05-27;12(6):. 2020-05-27 [PMID: 32471145] (Porcine)
• S Fourour, C Marois-Cré, L Martelet, C Fablet, I Kempf, M Gottschalk, M Segura Intra-Species and Inter-Species Differences in Cytokine Production by Porcine Antigen-Presenting Cells Stimulated by Mycoplasma hyopneumoniae, M. hyorhinis, and M. flocculare Pathogens, 2019-03-16;8(1):. 2019-03-16 [PMID: 30884861] (Porcine)
• CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs Planta, 2018-01-08;0(0):. 2018-01-08 [PMID: 29313103] (Porcine)
• Y Xiao, H Yan, H Diao, B Yu, J He, J Yu, P Zheng, X Mao, Y Luo, D Chen Early Gut Microbiota Intervention Suppresses DSS-Induced Inflammatory Responses by Deactivating TLR/NLR Signalling in Pigs Sci Rep, 2017-06-12;7(1):3224. 2017-06-12 [PMID: 28607413] (Porcine)
• De Clercq E, Devriendt B, Yin L, Chiers K, Cox E, Vanrompay D The immune response against Chlamydia suis genital tract infection partially protects against re-infection. Vet Res, 2014-09-25;45(0):95. 2014-09-25 [PMID: 25252649] (Porcine)
• Miller LC, Zanella EL, Waters WR Cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus. Clin. Vaccine Immunol., 2010-03-10;17(5):728-34. 2010-03-10 [PMID: 20219878] (Porcine)
• Poncelet AJ, Vercruysse J, Saliez A, Gianello P Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo. Transplantation, 2007-03-27;83(6):783-90. 2007-03-27 [PMID: 17414713] (Porcine)

FAQs for IL-12/IL-23 p40 (DY912). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.