POR/Cytochrome P450 Reductase Antibody

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP1-52083] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP1-52083] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POR/Cytochrome P450 Reductase Antibody Summary

• Description: Novus Biologicals Goat POR/Cytochrome P450 Reductase Antibody (NBP1-52083) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: internal region
• Epitope: C-TNPPRTNVLYE
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POR/Cytochrome P450 Reductase Antibody

• CPR
• CYPOR
• CYPORFLJ26468
• Cytochrome P450 Reductase
• DKFZp686G04235
• EC 1.6.2.4
• NADPH--cytochrome P450 reductase
• NADPH-dependent cytochrome P450 reductase
• P450 (cytochrome) oxidoreductase
• P450R
• POR

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POR
• Entrez:
  • 5447(Human)
• Uniprot:
  • NP_000932.3(Human)