POPDC3 Overexpression Lysate

Images

 
Western Blot: POPDC3 Overexpression Lysate (Adult Normal) [NBL1-14610] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POPDC3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POPDC3 Overexpression Lysate Summary

Description

POPDC3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204765

Accession#: NM_022361

Protein Tag: C-MYC/DDK
Gene
POPDC3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POPDC3 Overexpression Lysate

  • bA355M14.1
  • MGC22671
  • POP3RP11-99L11.2
  • popeye domain containing 3
  • popeye domain-containing protein 3
  • Popeye protein 3

Background

This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in these tissues during development. Alternatively spliced transcript variants have been found. [provided by RefSeq]. Transcript Variant: This variant (1) is the longer transcript and encodes a protein of 291 aa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol POPDC3