Human PON2 ELISA Kit (Colorimetric)

ELISA: Human PON2 ELISA Kit (Colorimetric) [NBP2-81263] - Samples were spiked with high concentrations of Human PON2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human PON2 ELISA Kit (Colorimetric) [NBP2-81263] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human PON2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human PON2 in samples. No significant cross-reactivity or interference between Human PON2 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.66%
• Intra-Assay: CV% < 4.77%
• Spike Recovery: 88-106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PON2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PON2 ELISA Kit (Colorimetric)

• A-esterase 2
• Aromatic esterase 2
• EC 3.1.1.2
• EC 3.1.1.81
• paraoxonase 2
• paraoxonase nirs
• PON 2
• PON2
• Serum Aryldialkylphosphatase 2
• serum paraoxonase/arylesterase 2

Background

PON2 encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PON2 ELISA Kit (NBP2-81263). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PON2