PON2 Antibody (JG35-80)

Western Blot: PON2 Antibody (JG35-80) [NBP2-75625] - Western blot analysis of PON2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: HCT116 cell lysate

Immunohistochemistry-Paraffin: PON2 Antibody (JG35-80) [NBP2-75625] - Analysis of paraffin-embedded mouse testis tissue using anti-PON2 antibody. Counter stained with hematoxylin.

Western Blot: PON2 Antibody (JG35-80) [NBP2-75625] - Analysis of PON2 on A549 cell lysate using anti-PON2 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: PON2 Antibody (JG35-80) [NBP2-75625] - Analysis of paraffin-embedded human colon cancer tissue using anti-PON2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PON2 Antibody (JG35-80) [NBP2-75625] - Analysis of paraffin-embedded human appendix tissue using anti-PON2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PON2 Antibody (JG35-80) [NBP2-75625] - Analysis of paraffin-embedded human kidney tissue using anti-PON2 antibody. Counter stained with hematoxylin.

Product Discontinued

PON2 Antibody (JG35-80) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within C-terminal Human PON2 aa 160-354 / 354. (SwissProt: Q15165 Human; SwissProt: Q62086 Mouse; SwissProt: Q6AXM8 Rat)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PON2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PON2 Antibody (JG35-80)

• A-esterase 2
• Aromatic esterase 2
• EC 3.1.1.2
• EC 3.1.1.81
• paraoxonase 2
• paraoxonase nirs
• PON 2
• PON2
• Serum Aryldialkylphosphatase 2
• serum paraoxonase/arylesterase 2

Background

PON2 encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PON2