Polypeptide GalNac Transferase 4/GALNT4 Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 4/GALNT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 4/GALNT4 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Polypeptide GalNac Transferase 4/GALNT4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 4/GALNT4
  Ala36-Lys578
  Accession # Q8N4A0
• Specificity: Detects human Polypeptide GalNac Transferase 4/GALNT4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GALNT4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Polypeptide GalNac Transferase 4/GALNT4 Antibody [Unconjugated]

• EC 2.4.1.41
• FLJ93314
• GALNACT4
• GalNAc-T4
• GALNT4
• Polypeptide GalNAc transferase 4
• polypeptide N-acetylgalactosaminyltransferase 4
• pp-GaNTase 4
• Protein-UDP acetylgalactosaminyltransferase 4
• UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
• UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 4 (GalNAc-T4)

Background

GALNT4 (Polypeptide N‑acetylgalactosaminyltransferase 4; also Polypeptide GalNAc transferase 4 and UDP-GalNAc:polypeptide Nacetylgalactosaminyltransferase 4) is a 66 kDa member of the GalNAC transferase subfamily, glycosyltransferase 4 family of enzymes. It is widely expressed, highly expressed in mucous cells. GALNT4 is found in the Golgi apparatus, and catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Human GALNT4 is a 578 amino acid (aa) type II transmembrane protein.  It contains a twelve aa N-terminal cytoplasmic region and a 543 aa extracellular lumenal domain (aa 36-578).  There is a potential 45 kDa splice form variant.  Over aa 36-578, human GALNT4 shares 92% aa sequence identity with mouse GALNT4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GALNT4
• Uniprot:
  • Q8N4A0(Human)