| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 3700-3860 of human PKD1 (NP_001009944.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PKD1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 462 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot: Polycystin 2 Antibody [NBP3-06363] - Anti-PKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDa](http://images.novusbio.com/images2/Polycystin-2-Antibody-Western-Blot-NBP3-06363-img0001.jpg)
![Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363] - PKD2 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PKD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-PKD2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size: 80 kDa](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunoprecipitation-NBP3-06363-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP3-06363] - Immunofluorescence staining of PKD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PKD2 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/images2/Polycystin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06363-img0003.jpg)
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.](http://images.novusbio.com/images/Protein-Kinase-D2-Antibody-(5G1)-Western-Blot-NBP2-02984-img0008.jpg)
![Flow Cytometry: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/Protein-Kinase-D2-Antibody-(5G1)-Flow-Cytometry-NBP2-02984-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.](http://images.novusbio.com/images2/Protein-Kinase-D2-Antibody-(5G1)-Immunocytochemistry-Immunofluorescence-NBP2-02984-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunocytochemistry-Immunofluorescence-H00005122-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Immunohistochemistry-Paraffin-H00005122-M02-img0012.jpg)
![Sandwich ELISA: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Detection limit for recombinant GST tagged PCSK1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/images2/Proprotein-Convertase-1-PCSK1-Antibody-(3D2)-Sandwich-ELISA-H00005122-M02-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/NHE6-SLC9A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38877-img0003.jpg)
![Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/images2/NHE6-SLC9A6-Antibody-Immunohistochemistry-Paraffin-NBP2-38877-img0006.jpg)
![Immunohistochemistry-Paraffin: NHE6/SLC9A6 Antibody [NBP2-38877] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/NHE6-SLC9A6-Antibody-Immunohistochemistry-Paraffin-NBP2-38877-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 6b Antibody [NBP2-14924] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Cytokeratin-6b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14924-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 6b Antibody [NBP2-14924] - Paraffin-embedded mouse brain, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Cytokeratin-6b-Antibody-Immunohistochemistry-Paraffin-NBP2-14924-img0002.jpg)
![Western Blot: Cytokeratin 6b Antibody [NBP2-14924] - Sample (1 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/images2/Cytokeratin-6b-Antibody-Western-Blot-NBP2-14924-img0003.jpg)
![ELISA: CBX4 Antibody (6C5G3) [NBP2-52432] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-ELISA-NBP2-52432-img0009.jpg)
![Flow Cytometry: CBX4 Antibody (6C5G3) [NBP2-52432] - Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-Flow-Cytometry-NBP2-52432-img0001.jpg)
![Immunohistochemistry-Paraffin: CBX4 Antibody (6C5G3) [NBP2-52432] - Analysis of cervical cancer tissues using CBX4 mouse mAb with DAB staining.](http://images.novusbio.com/images2/CBX4-Antibody-(6C5G3)-Immunohistochemistry-NBP2-52432-img0002.jpg)
![Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Flow-Cytometry-NBP2-22203-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg)
![Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/GDI1-Antibody-Western-Blot-NBP1-31528-img0002.jpg)
![Western Blot: GDI1 Antibody [NBP1-31528] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/GDI1-Antibody-Western-Blot-NBP1-31528-img0003.jpg)
![Immunohistochemistry-Paraffin: GDI1 Antibody [NBP1-31528] - Sample: Paraffin-embedded U87 xenograft. GDI1 antibody dilution: 1:200.](http://images.novusbio.com/images2/GDI1-Antibody-Immunohistochemistry-Paraffin-NBP1-31528-img0004.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Polycystin-1/PKD1 Antibody (NBP2-93178)Discover more about diseases related to Polycystin-1/PKD1 Antibody (NBP2-93178).
| Pathways for Polycystin-1/PKD1 Antibody (NBP2-93178)View related products by pathway.
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PTMs for Polycystin-1/PKD1 Antibody (NBP2-93178)Learn more about PTMs related to Polycystin-1/PKD1 Antibody (NBP2-93178).
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| Gene Symbol | PKD1 |
![Immunocytochemistry/Immunofluorescence: PKC mu Antibody [NBP1-87789] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/images2/PKC-mu-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87789-img0007.jpg)
![Immunohistochemistry-Paraffin: PKC mu Antibody [NBP1-87789] - Staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/PKC-mu-Antibody-Immunohistochemistry-Paraffin-NBP1-87789-img0006.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Thyroid Gland at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0002.jpg)
![Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.](http://images.novusbio.com/images2/ENPP-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27561-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-Immunohistochemistry-Paraffin-NBP2-38536-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human urinary bladder shows high expression.](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-Immunohistochemistry-Paraffin-NBP2-38536-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - Staining of human cell line hTCEpi shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/images2/Cytokeratin-17-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38536-img0007.jpg)
![Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Flow-Cytometry-NBP2-67360-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0003.jpg)
![Western Blot: PKD2L1 Antibody (4F9) [H00009033-M01] - PKD2L1 monoclonal antibody (M01), clone 4F9. Analysis of PKD2L1 expression in rat brain.](http://images.novusbio.com/images2/PKD2L1-Antibody-(4F9)-Western-Blot-H00009033-M01-img0001.jpg)
![Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.](http://images.novusbio.com/images2/Insulin-Receptor-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-12793-img0002.jpg)
![Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/images2/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NBP2-12793-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")