POLR3A Antibody - BSA Free

Western Blot: POLR3A Antibody - BSA Free [NBP3-45691] - MCF7 cells were subjected to SDS PAGE followed by western blot with (POLR3A antibody) at dilution of 1:300

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLR3A Antibody - BSA Free Summary

• Immunogen: polymerase(RNA) III(DNA directed) polypeptide A, 155kDa. (Uniprot# O14802)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 140-150 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for POLR3A Antibody - BSA Free

• DNA-directed RNA polymerase III largest subunit
• DNA-directed RNA polymerase III subunit A
• DNA-directed RNA polymerase III subunit RPC1
• EC 2.7.7.6
• hRPC155
• POL3A
• polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• RNA polymerase III 155 kDa subunit
• RNA polymerase III subunit C160
• RNA polymerase III subunit RPC155-D
• RPC1
• RPC155RNA polymerase III subunit C1

Background

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR3A