POLR2G Antibody

Western Blot: POLR2G Antibody [NBP1-37777] - Analysis of POLR2G in Human Brain (Cerebellum) lysates (calculated MW of 19.3kDa according to NP_002687.1) using NBP1-37777 at 1-3ug/ml.

• Reactivity: Hu, Mu, Rt, Sh
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POLR2G Antibody Summary

• Description: Novus Biologicals Goat POLR2G Antibody (NBP1-37777) is a polyclonal antibody validated for use in WB and ELISA. Anti-POLR2G Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EFDPNSNPPCYK corresponding to internal region according to NP_002687.1.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POLR2G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 21 kDa band observed in human brain (cerebellum) lysates (calculated MW of 19.3 kDa band according to NP_002687.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POLR2G Antibody

• DNA directed RNA polymerase II 19 kda polypeptide
• DNA-directed RNA polymerase II 19 kDa polypeptide
• DNA-directed RNA polymerase II subunit G
• DNA-directed RNA polymerase II subunit RPB7
• hRPB19
• hsRPB7
• MGC138369
• polymerase (RNA) II (DNA directed) polypeptide G
• RNA polymerase II 19 kDa subunit
• RNA polymerase II seventh subunit
• RNA polymerase II subunit B7
• RPB19
• RPB7MGC138367

Background

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, the association of this subunit with the polymerase under suboptimal growth conditions indicates it may play a role in regulating polymerase function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POLR2G Antibody (NBP1-37777)(1)

Publication using NBP1-37777

• Cojocaru M, Jeronimo C, Forget D et al. Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription. Biochem J 2008-01-01 [PMID: 17848138]

FAQs for POLR2G Antibody (NBP1-37777). (Showing 1 - 1 of 1 FAQ).

1. I found 4 antibodies against human POLR2G in your website: NBP1-32716, NBP1-37777, H00005436-B01 and H00005436-A01. Could you recommend one to do immunoprecipitation?
   • It looks like we have not validated immunoprecipitation for any of our POLR2G products. I therefore cannot offer any suggestion as to which product would be best suited for this assay. I can offer you our Innovator's Reward Program to any of the products you choose. In exchange for a review of our product in an application or species that has not been tested, we will issue you a credit for the purchase price of the antibody. See the following link for details: Innovator's Reward program. To help with your selection, I can say that the antibodies H00005436-B01 and H00005436-A01 are products that we distribute for a Taiwanese company called Abnova. We therefore do not do any in-house testing or development of their products. Abnova also does not offer any support for applications not listed on their datasheet. We can still offer you the Innovator's Reward on their products, but if you had any questions about the immunoprecipitation protocol or results, we would not be able to address them.

Bioinformatics

• Gene Symbol: POLR2G
• Entrez:
  • 5436(Human)
• Uniprot:
  • NP_002687.1(Human)