Recombinant Human Podocin/NPHS2 GST (N-Term) Protein

SDS-Page: Recombinant Human Podocin/NPHS2 Protein [H00007827-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human Podocin/NPHS2 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-315 of Human NPHS2 full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MERRARSSSRESRGRGGRTPHKENKRAKAERSGGGRGRQEAGPEPSGSGRAGTPGEPRAPAATVVDVDEVRGSGEEGTEVVALLESERPEEGTKSSGLGACEWLLVLISLLFIIMTFPFSIWFCVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Full Length Recombinant Protein
• Gene: NPHS2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 60.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Podocin/NPHS2 GST (N-Term) Protein

• Nephrosis 2
• nephrosis 2, idiopathic, steroid-resistant (podocin)
• NPHS2
• PDCN
• PDCNSRN1podocin
• Podocin
• SRN1

Background

This gene encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Podocin/NPHS2 Recombinant Protein (H00007827-P01)(3)

Publications using H00007827-P01

• Martineau, T;Boutin, M;Cote, AM;Maranda, B;Bichet, DG;Auray-Blais, C; Tandem mass spectrometry analysis of urinary podocalyxin and podocin in the investigation of podocyturia in women with preeclampsia and Fabry disease patients Clin. Chim. Acta 2019-03-19 [PMID: 30898510] (Human)
• Simon R, Lemoine J, Fonbonne C et al. Absolute quantification of podocin, a potential biomarker of glomerular injury in human urine, by Liquid Chromatography - Multiple Reaction Monitoring cubed Mass spectrometry. J Pharm Biomed Anal. 2014-01-24 [PMID: 24552645]
• Garovic VD, Craici IM, Wagner SJ et al. Mass spectrometry as a novel method for detection of podocyturia in pre-eclampsia. Nephrol Dial Transplant. 2012-04-20 [PMID: 22523117]

FAQs for Podocin/NPHS2 Recombinant Protein (H00007827-P01). (Showing 1 - 1 of 1 FAQs).

1. In the datasheet you have stated H00007827-P01 could be used for ELISA. Do you have any data to support this? Would the GST tag interfere with ELISA?
   • This product is made by a Taiwanese company called Abnova and we distribute it for them. They have only tested this protein for use as an ELISA control, and has only been validated for its use in ELISA with their antibodies.

Bioinformatics

• Gene Symbol: NPHS2