Recombinant Human PNPO His Protein

SDS-Page: Recombinant Human PNPO His Protein [NBP1-50887] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human PNPO His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 57-261 of Human PNPO
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PNPO
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PNPO His Protein

• EC 1.4.3.5
• FLJ10535
• PDXPO
• pyridoxal 5'-phosphate synthase
• pyridoxamine 5'-phosphate oxidase
• Pyridoxamine-phosphate oxidase
• pyridoxine 5'-phosphate oxidase
• pyridoxine-5'-phosphate oxidase

Background

PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PNPO