PMP70 Antibody (CL2524) - Azide and BSA Free

Staining in HeLa cell line with Anti-PMP70 monoclonal antibody) .

Staining in U251 cell line with Anti-PMP70 monoclonal antibody) .

Staining in MCF7 cell line with Anti-PMP70 monoclonal antibody) .

Staining in A431 cell line with Anti-PMP70 monoclonal antibody) .

Analysis in human liver and pancreas tissues using NBP3-44465 antibody. Corresponding PMP70 RNA-seq data are presented for the same tissues.

Staining in U2OS cell line with Anti-PMP70 monoclonal antibody) .

Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.

Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.

Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.

Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL2524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PMP70 Antibody (CL2524) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PMP70 Antibody (CL2524) - Azide and BSA Free (NBP2-36770) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P28288, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCD3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PMP70 Antibody (CL2524) - Azide and BSA Free

• 70 kDa peroxisomal membrane protein
• ABC43
• ATP-binding cassette, sub-family D (ALD), member 3
• peroxisomal membrane protein 1 (70kD, Zellweger syndrome)
• Peroxisomal membrane protein-1 (70kD)
• PMP70ATP-binding cassette sub-family D member 3
• PXMP1dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1))
• ZWS2

Background

PMP70 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABCD3