PMEL17/SILV Antibody (OTI10E5)

Images

 
Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody (10E5) [NBP1-47974] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded breast tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (10E5) [NBP1-47974] - Staining of paraffin-embedded Kidney tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI10E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.2 mg/ml

Order Details

PMEL17/SILV Antibody (OTI10E5) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SILV expression vector
Marker
Melanosome Marker
Specificity
This antibody is specific for Homo sapiens silver homolog (mouse) (SILV).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
Theoretical MW
70.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47974 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PMEL17/SILV Antibody (OTI10E5)

  • D12S53EP1
  • Gp100
  • ME20
  • ME20M
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL
  • PMEL17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • SILV
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Background

Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for PMEL17/SILV Antibody (NBP1-47974) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47974:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry PMEL17/SILV NBP1-47974
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5
reviewed by:
Verified Customer
ICC Human 09/05/2021
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Summary

ApplicationImmunocytochemistry
Sample TestedHeLa cells
SpeciesHuman
LotOTI10E5

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP1-47974) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
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1
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Verified Customer
09/05/2021
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PMEL
Entrez
Uniprot