PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

Western Blot: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV

Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded Kidney tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded breast tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.

Immunohistochemistry: PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free [NBP2-73479] - Staining of paraffin-embedded prostate tissue using anti-PMEL17/SILV mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI10E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free (NBP1-47974) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human SILV expression vector
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:500
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 70.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PMEL17/SILV Antibody (OTI10E5) - Azide and BSA Free

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMEL