PMCA2 Antibody

Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Cancer biopsies of deparaffinized Human cervical carcinoma tissue.

• Reactivity: Hu, Rt, Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PMCA2 Antibody Summary

• Immunogen: Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)
• Specificity: PMCA2 antibody detects calcium pump of the plasma membrane 2 (PMCA2 ATPase) from human and rat and porcine tissues and cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 127 kDa and 133 kDa proteins representing PMCA 2a and 2b ATPase from rat brain microsomal fractions.
• Control:

  Human Brain Cerebellum Membrane Tissue Lysate (Adult Membrane Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMCA2 Antibody

• plasma membrane calcium-transporting ATPase 2
• ATPase, Ca++ transporting, plasma membrane 2
• PMCA2
• PMCA2a
• PMCA2i

Background

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2B2
• Entrez:
  • 491(Human)
  • 24215(Rat)
• Uniprot:
  • Q01814(Human)
  • P11506(Rat)