PMCA3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PMCA3 Antibody [NBP1-87259] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-87259 antibody. Corresponding PMCA3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human cerebellum shows strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PMCA3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IRVVKAFRSSLYEGLEKPESKTSIHNFMATPEFLINDYTHNIPLIDDTDVDENEERLRAPPPPSPNQNNNAIDSGIYLTTHVTKSATSSVFSSSPGSPLHSVETS
Specificity
Specificity of human PMCA3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP2B3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PMCA3 Protein (NBP1-87259PEP)
Publications
Read Publication using NBP1-87259.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23416519)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PMCA3 Antibody

  • ATPase, Ca++ transporting, plasma membrane 3
  • EC 3.6.3
  • EC 3.6.3.8
  • Plasma membrane calcium ATPase isoform 3
  • plasma membrane calcium ATPase
  • Plasma membrane calcium pump isoform 3
  • plasma membrane calcium-transporting ATPase 3
  • PMCA3a
  • PMCA3plasma membrane calcium pump

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PMCA3 Antibody (NBP1-87259)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PMCA3 Antibody (NBP1-87259). (Showing 1 - 2 of 2 FAQ).

  1. I would like to know if this antibody can be stained the fixed sample by using aceton-methanol.
    • For our product NBP1-87259, this antibody is made to the cytoplasmic domain of the PMCA3 protein. For our ICC staining, we fix the cells with 4% paraformaldehyde pH 7.2 - 7.3 in growth medium supplemented with 10% fetal bovine serum (FBS) for 15 minutes on ice. It is possible that a acetone-methanol fixation may also work as the antibody will detect an epitope on the cytoplasmic side of the cell membrane.
  2. And, what kind of fix solution did your IF data use?
    • I would recommend using the paraformaldehyde fixation if the results are inconclusive with the acetone-methanol fixation.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional PMCA3 Products

Array NBP1-87259

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Bioinformatics

Gene Symbol ATP2B3