SERCA3 ATPase Antibody (2H3)

Western Blot: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - ATP2A3 monoclonal antibody (M01), clone 2H3 Analysis of ATP2A3 expression in HL-60.

Immunohistochemistry-Paraffin: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - Analysis of monoclonal antibody to ATP2A3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Sandwich ELISA: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - Detection limit for recombinant GST tagged ATP2A3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC-P
• Clone: 2H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SERCA3 ATPase Antibody (2H3) Summary

• Immunogen: ATP2A3 (AAH35729, 501 a.a. - 620 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TRPHPTGQGSKMFVKGAPESVIERCSSVRVGSRTAPLTPTSREQILAKIRDWGSGSDTLRCLALATRDAPPRKEDMELDDCSKFVQYETDLTFVGCVGMLDPPRPEVAACITRCYQAGIR
• Specificity: ATP2A3 - ATPase, Ca++ transporting, ubiquitous
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP2A3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Immunohistochemistry-Paraffin
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SERCA3 ATPase Antibody (2H3)

• adenosine triphosphatase, calcium
• ATPase, Ca(2+)-transporting, ubiquitous
• ATPase, Ca++ transporting, ubiquitous
• Calcium pump 3
• EC 3.6.3
• EC 3.6.3.8
• sarco/endoplasmic reticulum Ca2+ ATPase
• sarco/endoplasmic reticulum Ca2+ -ATPase
• sarcoplasmic/endoplasmic reticulum calcium ATPase 3
• SERCA3calcium-translocating P-type ATPase
• SR Ca(2+)-ATPase 3

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SERCA3 ATPase Antibody (H00000489-M01)(3)

Publications using H00000489-M01

• Ait-Ghezali L, Arbabian A, Jeibmann A et al. Loss of endoplasmic reticulum calcium pump expression in choroid plexus tumours. Neuropathol Appl Neurobiol. 2014 Oct [PMID: 24224513] (Human)
• Arbabian A, Brouland JP, Apati A et al. Modulation of endoplasmic reticulum calcium pump expression during lung cancer cell differentiation. FEBS J. 2012 Nov 15 [PMID: 23157274]
• Corvazier E, Bredoux R, Kovacs T, Enouf J. Expression of Sarco/Endoplasmic Reticulum Ca2+ATPase (SERCA) 3 proteins in two major conformational states in native human cell membranes. Biochim Biophys Acta. 2008 Dec 01 [PMID: 19135027]

Bioinformatics

• Gene Symbol: ATP2A3
• Entrez:
  • 489(Human)
• OMIM:
  • 601929(Human)
• Uniprot:
  • AAH35729(Human)