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SERCA3 ATPase Antibody (2H3)

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Western Blot: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - ATP2A3 monoclonal antibody (M01), clone 2H3 Analysis of ATP2A3 expression in HL-60.
Western Blot: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - ATP2A3 monoclonal antibody (M01), clone 2H3 Analysis of ATP2A3 expression in HL-60.
Immunohistochemistry-Paraffin: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - Analysis of monoclonal antibody to ATP2A3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
ELISA: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - Detection limit for recombinant GST tagged ATP2A3 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SERCA3 ATPase Antibody (2H3) Summary

Immunogen
ATP2A3 (AAH35729, 501 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TRPHPTGQGSKMFVKGAPESVIERCSSVRVGSRTAPLTPTSREQILAKIRDWGSGSDTLRCLALATRDAPPRKEDMELDDCSKFVQYETDLTFVGCVGMLDPPRPEVAACITRCYQAGIR
Specificity
ATP2A3 - ATPase, Ca++ transporting, ubiquitous
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ATP2A3
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using H00000489-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SERCA3 ATPase Antibody (2H3)

  • adenosine triphosphatase, calcium
  • ATPase, Ca(2+)-transporting, ubiquitous
  • ATPase, Ca++ transporting, ubiquitous
  • Calcium pump 3
  • EC 3.6.3
  • EC 3.6.3.8
  • sarco/endoplasmic reticulum Ca2+ ATPase
  • sarco/endoplasmic reticulum Ca2+ -ATPase
  • sarcoplasmic/endoplasmic reticulum calcium ATPase 3
  • SERCA3calcium-translocating P-type ATPase
  • SR Ca(2+)-ATPase 3

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Western Blot: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3).
Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.
Species: Hu, Mu, Rt, Am, Ca, Rb
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Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
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Western Blot: Phospholamban Antibody [NBP2-19807] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP2-19807] - Human colon carcinoma, using phospholamban antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Cancer biopsies of deparaffinized Human cervical carcinoma tissue.
Species: Hu, Rt, Po
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
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Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.
Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
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Western Blot: HOXA10 Antibody [NBP3-04877] - Analysis of extracts of Mouse kidney, using HOxA10 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit
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Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY licence.
Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - Analysis of MAM-associated protein levels in control and patients' fibroblasts.(A) Equal amounts from one control and the five patients were loaded (50 ug of total cell lysates) and subjected to Western Blot with anti-Grp75, anti-sigma-1R and anti-Mfn2 antibodies. We used anti-GAPDH antibody to ensure equal amounts of protein loaded in each lane. This result is representative of three independent experiments. PLoS One. 2016 Mar 9;11(3):e0150357. doi: 10.1371/journal.pone.0150357
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Western Blot: gamma-Synuclein Antibody [NBP1-31209] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma-Synuclein antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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Immunohistochemistry-Paraffin: TCF7L2 Antibody (3A4) [H00006934-M06] - Analysis of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
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Chromatin Immunoprecipitation: SP1 Antibody [NB600-233] - Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of Sp1 binding within a 250 Kb region of chromosome 6 asdetected using anti-Sp1.
(4 Publications)
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
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2 Antibody
NBP2-76961
Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 2 Antibody [NBP2-76961] - ICC staining RYR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Ryanodine Receptor 2 Antibody [NBP2-76961] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR2 antibody. Counter stained with hematoxylin.
Flow Cytometry: Ryanodine Receptor 2 Antibody [NBP2-76961] - Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for SERCA3 ATPase Antibody (H00000489-M01)(3)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol ATP2A3
Entrez
OMIM
Uniprot