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![Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/PMCA2-Antibody-Immunohistochemistry-Paraffin-NB120-3529-img0004.jpg "Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.")
![Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Cancer biopsies of deparaffinized Human cervical carcinoma tissue.](http://images.novusbio.com/fullsize/PMCA2-Antibody-Immunohistochemistry-Paraffin-NB120-3529-img0005.jpg)
![Western Blot: PMCA2 Antibody [NBP3-41513] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-41513_rabbit-pmca2-pab-612202421121394.jpg "Western Blot: PMCA2 Antibody [NBP3-41513] - Sample: Recombinant protein.")
![Immunohistochemistry-Paraffin: PMCA2 Antibody [NBP3-41513] - DAB staining on IHC-P; Samples: Mouse Heart Tissue; Primary Ab: 20ug/ml NBP3-41513; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41513_rabbit-pmca2-pab-612202421175958.jpg)
![Immunohistochemistry-Paraffin: PMCA2 Antibody [NBP3-41513] - DAB staining on fromalin fixed paraffin-embedded brain tissue](http://images.novusbio.com/fullsize/nbp3-41513_rabbit-pmca2-pab-61220242151895.jpg)
![Immunohistochemistry: PMCA2 Antibody - BSA Free [NBP3-47108] - Immunohistochemistry of paraffin-embedded human brain using (PMCA2 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-47108_rabbit-pmca2-pab-bsa-free-284202512343869.jpg "Immunohistochemistry: PMCA2 Antibody - BSA Free [NBP3-47108] - Immunohistochemistry of paraffin-embedded human brain using (PMCA2 antibody) at dilution of 1:100")
![Western Blot: PMCA2 Antibody - BSA Free [NBP3-47108] - Human brain tissue were subjected to SDS PAGE followed by western blot with (PMCA2 antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-47108_rabbit-pmca2-pab-bsa-free-28420251231232.jpg)
![Western Blot: PMCA2 Antibody [NBP2-95221] - Western blot analysis of extracts of SH-SY5Y cells, using PMCA2 antibody (NBP2-95221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PMCA2 Antibody-Western Blot-NBP2-95221-img0002.jpg "Western Blot: PMCA2 Antibody [NBP2-95221] - Western blot analysis of extracts of SH-SY5Y cells, using PMCA2 antibody (NBP2-95221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")