PMCA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.

Western Blot: PMCA1 Antibody [NBP1-87261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human Cerebral Cortex tissue.

Immunocytochemistry/Immunofluorescence: PMCA1 Antibody [NBP1-87261] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus & plasma membrane.

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human kidney shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PMCA1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGI
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PMCA1 Protein (NBP1-87261PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PMCA1 Antibody - BSA Free

• ATPase, Ca++ transporting, plasma membrane 1
• EC 3.6.3
• EC 3.6.3.8
• Plasma membrane calcium ATPase isoform 1
• plasma membrane calcium ATPase
• Plasma membrane calcium pump isoform 1
• plasma membrane calcium pump
• plasma membrane calcium-ATPase
• PMCA1kb
• PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP2B1