Hu,
Mu,
Rt,
Po,
Am,
Ca,
Ch,
Fe,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, Sh
WB, Flow, ICC/IF, IHC, IP
Applications: WB, Flow, ICC/IF, IHC, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Western-Blot-NB300-578-img0015.jpg "Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).")
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0014.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0005.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0012.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0013.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0010.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PMCA1 Antibody [NBP1-87261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human Cerebral Cortex tissue.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP1-87261-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody [NBP1-87261] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus & plasma membrane.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87261-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0008.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0009.jpg)
![Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP3-12964-img0001.jpg "Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: PMCA1 protein stained by PMCA1 antibody (NBP3-12964) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0002.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0003.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PMCA1 Antibody [NBP1-87260] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP1-87260-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0005.jpg)
![Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830] - Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/1,000 dilution. Lane 1: A549 cell lysate (no heat) Lane 2: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 135 kDa Observed band size: 135-250 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32830_rabbit-pmca1-mab-je34-05-288202412442991.jpg "Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830] - Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/1,000 dilution. Lane 1: A549 cell lysate (no heat) Lane 2: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 135 kDa Observed band size: 135-250 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMCA1 Antibody (JE34-05) [NBP3-32830] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32830) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32830_rabbit-pmca1-mab-je34-05-29820241435250.jpg)
![Western Blot: PMCA1 Antibody [NBP1-97754] - (0.03ug/ml) Analysis of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP1-97754-img0002.jpg "Western Blot: PMCA1 Antibody [NBP1-97754] - (0.03ug/ml) Analysis of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PMCA1 Antibody (S02-8C5) [NBP3-19742] - Western blot detection of PMCA1 in U87-MG, human placenta lysates using PMCA1 antibody. Predicted band size: 139kDa. Observed band size: 130-250kDa.](http://images.novusbio.com/fullsize/PMCA1-Antibody-S02-8C5-Western-Blot-NBP3-19742-img0001.jpg "Western Blot: PMCA1 Antibody (S02-8C5) [NBP3-19742] - Western blot detection of PMCA1 in U87-MG, human placenta lysates using PMCA1 antibody. Predicted band size: 139kDa. Observed band size: 130-250kDa.")
![SDS-Page: Recombinant Human PMCA1 Protein [H00000490-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-PMCA1-Protein-SDS-Page-H00000490-Q01-img0001.jpg "SDS-Page: Recombinant Human PMCA1 Protein [H00000490-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Sandwich ELISA: PMCA1 Antibody (3E2) [H00000490-M01] - Detection limit for recombinant GST tagged ATP2B1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PMCA1-Antibody-3E2-Sandwich-ELISA-H00000490-M01-img0002.jpg "Sandwich ELISA: PMCA1 Antibody (3E2) [H00000490-M01] - Detection limit for recombinant GST tagged ATP2B1 is approximately 0.3ng/ml as a capture antibody.")