PLZF Antibody (816416)

Western blot shows lysates of BaF3 mouse pro-B cell line and KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse PLZF Monoclonal Antibody (Catalog # MAB8395) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLZF at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 816416
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PLZF Antibody (816416) Summary

• Immunogen: E. coli-derived recombinant mouse PLZF
  Met1-Gln254
  Accession # NP_001028496
• Specificity: Detects mouse PLZF in direct ELISA and Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ZBTB16
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLZF Antibody (816416)

• PLZF
• PLZFzinc finger and BTB domain-containing protein 16
• ZBTB16
• zinc finger and BTB domain containing 16
• zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
• Zinc finger protein 145
• Zinc finger protein PLZF
• ZNF145
• ZNF145Promyelocytic leukemia zinc finger protein

Background

Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB16
• Uniprot:
  • NP_001028496()