PLOD3 Antibody

Images

 
Western Blot: PLOD3 Antibody [H00008985-B01P] - Analysis of PLOD3 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: PLOD3 Antibody [H00008985-B01P] - Analysis of purified antibody to PLOD3 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: PLOD3 Antibody [H00008985-B01P] - Analysis of PLOD3 expression in transfected 293T cell line by PLOD3 polyclonal antibody. Lane 1: PLOD3 transfected lysate(81.18 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PLOD3 Antibody Summary

Immunogen
PLOD3 (NP_001075.1, 1 a.a. - 738 a.a.) full-length human protein. MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
Specificity
PLOD3 - procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PLOD3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.
Control
PLOD3 Lysate (NBP2-66036)
Publications
Read Publications using H00008985-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLOD3 Antibody

  • 2-oxoglutarate 5-dioxygenase 3
  • EC 1.14.11.4
  • LH3Lysyl hydroxylase 3
  • lysine hydroxylase 3
  • procollagen-lysine
  • procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Background

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLOD3 Antibody (H00008985-B01P)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLOD3
Entrez
Uniprot