PLOD3 Antibody - Azide and BSA Free

Western Blot: PLOD3 Antibody [H00008985-B01P] - Analysis of PLOD3 expression in HepG2.

Immunocytochemistry/Immunofluorescence: PLOD3 Antibody [H00008985-B01P] - Analysis of purified antibody to PLOD3 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: PLOD3 Antibody [H00008985-B01P] - Analysis of PLOD3 expression in transfected 293T cell line by PLOD3 polyclonal antibody. Lane 1: PLOD3 transfected lysate(81.18 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PLOD3 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PLOD3 (NP_001075.1, 1 a.a. - 738 a.a.) full-length human protein. MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
• Specificity: PLOD3 - procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PLOD3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLOD3 Antibody - Azide and BSA Free

• 2-oxoglutarate 5-dioxygenase 3
• EC 1.14.11.4
• LH3Lysyl hydroxylase 3
• lysine hydroxylase 3
• procollagen-lysine
• procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Background

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLOD3 Antibody (H00008985-B01P)(2)

Publications using H00008985-B01P

• Amodio G, Sasso E, D'Ambrosio C et al. Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach. Cell Biol Toxicol 2016-05-27 [PMID: 27233793]
• Amodio G, Moltedo O, Monteleone F et al. Proteomic Signatures in Thapsigargin-Treated Hepatoma Cells. Chem Res Toxicol. 2011-07-01 [PMID: 21692457]

Bioinformatics

• Gene Symbol: PLOD3
• Entrez:
  • 8985(Human)
• Uniprot:
  • NP_001075.1(Human)