PLOD3 Antibody (2B1) - BSA Free

Western Blot: PLOD3 Antibody (2B1) [NBP3-43020] - human placenta tissue were subjected to SDS PAGE followed by western blot with (PLOD3 antibody) at dilution of 1:300

Immunohistochemistry: PLOD3 Antibody (2B1) [NBP3-43020] - Immunohistochemistry of paraffin-embedded human pancreas cancer using (PLOD3 antibody) at dilution of 1:100

Immunoprecipitation: PLOD3 Antibody (2B1) [NBP3-43020] - IP Result of anti-PLOD3 (IP:FNab06554, 5ug; Detection:FNab06554 1:500) with HeLa cells lysate 1400ug.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 2B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PLOD3 Antibody (2B1) - BSA Free Summary

• Description: Novus Biologicals Mouse PLOD3 Antibody (2B1) - BSA Free (NBP3-43020) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 - (Uniprot# O60568)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLOD3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:20 - 1:200
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for PLOD3 Antibody (2B1) - BSA Free

• 2-oxoglutarate 5-dioxygenase 3
• EC 1.14.11.4
• LH3Lysyl hydroxylase 3
• lysine hydroxylase 3
• procollagen-lysine
• procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3

Background

PLOD3 is encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLOD3