Plexin B3 Antibody (905805)

Images

 
A172 human glioblastoma cell line was stained with Mouse Anti-Human Plexin B3 Monoclonal Antibody (Catalog # MAB4958, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
905805
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Plexin B3 Antibody (905805) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Plexin B3
His45-Gln1255
Accession # Q9ULL4
Specificity
Detects human Plexin B3 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLXNB3
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B3 Antibody (905805)

  • PLEXB3
  • Plexin B3
  • plexin-B3
  • PLEXR
  • PLXN6
  • PLXNB3

Background

Plexin B3 is a type I transmembrane glycoprotein that belongs to the Plexin B subfamily of semaphorin receptors (1, 2). Human Plexin B3 contains a 44 amino acid (aa) signal sequence, a 1211 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other Plexins, such as an LRR (C1) and LRF (C2) domain, and a PDZ motif common to B Plexins (1). The PDZ motif is thought to be involved in RhoA activation and axonal growth cone collapse downstream of semaphorin engagement (3). The human Plexin B3 ECD shares 81%, 81%, 83% and 75% aa identity with mouse, rat, bovine and canine Plexin B3, respectively. It contains a sema domain and a Plexin-Semaphorin-Integrin (PSI) or Met-Related Sequence (MRS) domain, each of which contains a potential proteolytic cleavage site (2). Detection of 160 and 140 kDa species along with the full-length 260 kDa Plexin B3 in homogenates of human neocortex indicates that proteolytic processing may occur in vivo (2). The ECD also contains four glycine/proline-rich IPT/TIG domains, which are immunoglobulin-like domains found in Plexins, transcription factors, and the scatter factor receptors Met and Ron. B Plexins, including Plexin B3, can interact with Met and Ron, activating these receptors upon semaphorin engagement (4, 5). Plexin B3 and its identified ligand, the transmembrane semaphorin Sema5A, are both expressed during differentiation and migration of central nervous system oligodendrocytes (5‑7). However, the developmental functions of Sema5A are likely independent of Plexin B3, which is not significantly expressed prenatally (8). In turn, Plexin B3 may exhibit Sema5A-independent activity, as homophilic interactions of sema domains are reported to stimulate neurite outgrowth of postnatal cerebellar neurons (2).

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Hartwig, C. et al. (2005) BMC Neurosci. 6:53.
  3. Swiercz, J.M. et al. (2002) Neuron 35:51.
  4. Conrotto, P. et al. (2004) Oncogene 23:5131.
  5. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  6. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  7. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  8. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4958U
Alexa Fluor 405 FAB4958V
Alexa Fluor 488 FAB4958G
Alexa Fluor 594 FAB4958T
Alexa Fluor 647 FAB4958R
Alexa Fluor 700 FAB4958N
Alexa Fluor 750 FAB4958S

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Bioinformatics

Gene Symbol PLXNB3
Uniprot