Plexin B3 Antibody (905805) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
905805
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Plexin B3 Antibody (905805) [Alexa Fluor® 750] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Plexin B3
His45-Gln1255
Accession # Q9ULL4
Specificity
Detects human Plexin B3 in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLXNB3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B3 Antibody (905805) [Alexa Fluor® 750]

  • PLEXB3
  • Plexin B3
  • plexin-B3
  • PLEXR
  • PLXN6
  • PLXNB3

Background

Plexin B3 is a type I transmembrane glycoprotein that belongs to the Plexin B subfamily of semaphorin receptors (1, 2). Human Plexin B3 contains a 44 amino acid (aa) signal sequence, a 1211 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other Plexins, such as an LRR (C1) and LRF (C2) domain, and a PDZ motif common to B Plexins (1). The PDZ motif is thought to be involved in RhoA activation and axonal growth cone collapse downstream of semaphorin engagement (3). The human Plexin B3 ECD shares 81%, 81%, 83% and 75% aa identity with mouse, rat, bovine and canine Plexin B3, respectively. It contains a sema domain and a Plexin-Semaphorin-Integrin (PSI) or Met-Related Sequence (MRS) domain, each of which contains a potential proteolytic cleavage site (2). Detection of 160 and 140 kDa species along with the full-length 260 kDa Plexin B3 in homogenates of human neocortex indicates that proteolytic processing may occur in vivo (2). The ECD also contains four glycine/proline-rich IPT/TIG domains, which are immunoglobulin-like domains found in Plexins, transcription factors, and the scatter factor receptors Met and Ron. B Plexins, including Plexin B3, can interact with Met and Ron, activating these receptors upon semaphorin engagement (4, 5). Plexin B3 and its identified ligand, the transmembrane semaphorin Sema5A, are both expressed during differentiation and migration of central nervous system oligodendrocytes (5‑7). However, the developmental functions of Sema5A are likely independent of Plexin B3, which is not significantly expressed prenatally (8). In turn, Plexin B3 may exhibit Sema5A-independent activity, as homophilic interactions of sema domains are reported to stimulate neurite outgrowth of postnatal cerebellar neurons (2).

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Hartwig, C. et al. (2005) BMC Neurosci. 6:53.
  3. Swiercz, J.M. et al. (2002) Neuron 35:51.
  4. Conrotto, P. et al. (2004) Oncogene 23:5131.
  5. Artigiani, S. et al. (2004) EMBO Rep. 5:710.
  6. Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
  7. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  8. Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLXNB3
Entrez
Uniprot