Plexin A2 Antibody (583603) [Unconjugated]

Western blot shows lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 583603
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Plexin A2 Antibody (583603) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
  Met35-Pro1237
  Accession # P70207
• Specificity: Detects mouse Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B2, D1, recombinant mouse Plexin A1, A3, B3, or C1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: PLXNA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A2 Antibody (583603) [Unconjugated]

• FLJ11751
• FLJ30634
• KIAA0463plexin 2
• OCT
• OCTSemaphorin receptor OCT
• Plexin A2
• PLXN2
• PLXN2plexin-A2
• PLXNA2
• transmembrane protein OCT

Background

Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells. It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There is one alternate start site 10 aa upstream of the standard start site. Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin A2 Antibody (MAB5486)(1)

Publication using MAB5486

• Tian T, Tomavo N, Huot L, Flourens A, Bonnelye E, Flajollet S, Hot D, Leroy X, de Launoit Y, Duterque-Coquillaud M Identification of novel TMPRSS2:ERG mechanisms in prostate cancer metastasis: involvement of MMP9 and PLXNA2. Oncogene, 2013-05-27;33(17):2204-14. 2013-05-27 [PMID: 23708657] (IHC, Human)

Bioinformatics

• Gene Symbol: PLXNA2
• Uniprot:
  • P70207()