PLET-1 Antibody (700738) [Unconjugated]

Mouse thymocytes were stained with Rat Anti-Mouse PLET‑1 Monoclonal Antibody (Catalog # MAB6917, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

PLET-1 was detected in immersion fixed frozen sections of adult mouse kidney using Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to medulla collecting ducts. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 700738
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PLET-1 Antibody (700738) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PLET-1
  Ala27-Leu216
  Accession # Q8VEN2
• Specificity: Detects mouse PLET-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Plet1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLET-1 Antibody (700738) [Unconjugated]

• AgK114
• C11orf34
• chromosome 11 open reading frame 34
• placenta expressed transcript 1
• PLET1
• PLET-1

Background

PLET-1 (placenta-expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI-anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET-1, which most likely includes amino acids (aa) 28-216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the C‑terminus. PLET-1 is a marker of early thymic progenitor cells and pancreatic duct epithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLET-1 Antibody (MAB6917)(1)

Publication using MAB6917

• Pervizaj-Oruqaj, L;Selvakumar, B;Ferrero, MR;Heiner, M;Malainou, C;Glaser, RD;Wilhelm, J;Bartkuhn, M;Weiss, A;Alexopoulos, I;Witte, B;Gattenlöhner, S;Vadász, I;Morty, RE;Seeger, W;Schermuly, RT;Vazquez-Armendariz, AI;Herold, S; Alveolar macrophage-expressed Plet1 is a driver of lung epithelial repair after viral pneumonia Nature communications 2024-01-02 [PMID: 38167746] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Plet1
• Uniprot:
  • Q8VEN2()